Restriction Enzymes
New England Biolabs, Inc. BsaAI – 500 units
A restriction endonuclease that recognizes the sequence YAC_^GTR.
New England Biolabs, Inc. AseI – 10000 units
A restriction endonuclease that recognizes the sequence AT^TA_AT.
New England Biolabs, Inc. Cac8I – 500 units
A restriction endonuclease that recognizes the sequence GCN_^NGC.
New England Biolabs, Inc. BmrI – 100 units
A restriction endonuclease that recognizes the sequence ACTGGGNNNN_N^.
New England Biolabs, Inc. PflFI – 2000 units
A restriction endonuclease that recognizes the sequence GACN^N_NGTC.
New England Biolabs, Inc. BseYI – 500 units
A restriction endonuclease that recognizes the sequence C^CCAG_C.
New England Biolabs, Inc. PshAI – 1000 units
A restriction endonuclease that recognizes the sequence GACNN_^NNGTC.
New England Biolabs, Inc. BspEI – 1000 units
A restriction endonuclease that recognizes the sequence T^CCGG_A.
New England Biolabs, Inc. EagI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. MspI – 25000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. ApaI – 5000 units
A restriction endonuclease that recognizes the sequence G_GGCC^C.
New England Biolabs, Inc. BstXI – 1000 units
A restriction endonuclease that recognizes the sequence CCAN_NNNN^NTGG.
New England Biolabs, Inc. HindIII – 10000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. FokI – 1000 units
A restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_.
New England Biolabs, Inc. HaeII – 10000 units
A restriction endonuclease that recognizes the sequence R_GCGC^Y.